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Given that CHOP is a well-established pro-apoptotic protein , it could very well be a key driver of the synergistic cell death that we observed in this context. To experimentally verify this prediction, first, we treated a glioblastoma cell line T98G with different concentrations of CFZ in combination with each of the BET inhibitors JQ1, I-BET762, and I-BET151. Then, we analyzed the resultant cell viability data using the established Chou-Talalay method, wherein a combination index value less than 1.0 is regarded synergistic .
Given that the fraction affected is a measure of cell viability, we considered Fa values greater than 0.75 to be optimal. Using these criteria, we found several optimal CFZ + BET inhibitor combinations that were highly synergistic in the T98G cell line (Figure 1B; first panel). In order to test if this effect is true for cell lines derived from other tumor types, we employed A549 , HCT116 , MDA-MB-231 , DU145 , and MIAPaCa2 cell lines in a similar experiment. Indeed, we could find several optimal CFZ + BET inhibitor synergistic combinations in all of these cell lines (Figure 1B; panels 2–6), implying that this could be a general phenomenon independent of cancer type.
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Cells were treated with 4 concentrations of CFZ each in combination with 4 combinations of BET inhibitors in four-fold increments, as indicated above. This yielded a total of 16 different combinations and 10 different ratios. Each drug was also treated alone at these concentrations. All the treatments were done in triplicates. Fa was calculated for all individual and cotreatments and used to determine CI using CompuSyn software (ComboSyn Inc., Paramus, NJ, USA) which employs the Chou–Talalay method .
The clinical utility of the PCR test for the tested persons is zero, otherwise such tests would have been in use for at least a decade to support diagnosis and therapy of influenza or heavy cold cases. We have answered Mäuschen extensively on her confusing thread, and also invited her to participate at the discussion directly here with all the authors. She didn’t follow the invitation until yet. All her points are directly refuted and thoroughly debunked several times in her thread, it seems like she has no clue about the things she claims to have any kind of expertise. And not supporting anything about specificity but only sensitivity of the dilution series of this one specific sample. 5) We know that most of the western hemisphere uses 2 confirmatory assays.
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Three confirmatory assay-steps would at least minimize-out errors & uncertainties at every fold-step in regards to “Wobbly”-spots. (Nonetheless, the protocol would still fall short of any “good laboratory practice”, when factoring in all the other design-errors). With the help of our expert scientists and engineers, we have been able to provide you excellent services of Bacterial Endotoxin Test . We implement best available quantitative turbidimetric assay techniques in a designated area of our microbiological laboratory to perform Bacterial Endotoxin Test . We conduct different types of testing and analysis including analytical testing, stability testing and microbiology services to offer exact result.
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